Composite

Part:BBa_K781007:Design

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-03)


[R0010][B0034] - XylE-FliC Deletion Chimera


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2130
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1118
    Illegal NgoMIV site found at 1290
    Illegal AgeI site found at 529
    Illegal AgeI site found at 1641
    Illegal AgeI site found at 1726
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When amplifying this part, the overlapping regions were carefully chosen so that the insert sequence alone can be inforporated into a plasmid, for future use in overlap extension. Digesting with the two inner enzymes for the BB-2 enzymes S and N, leaves the appropriate sequence for overlap extension.


Source

The original XylE came from a biobrick part. It was amplified using PCR and then overlap extension was used to incorporate it into the flagella.

References